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Issue Environ. Biosafety Res.
Volume 8, Number 2, April-June 2009
Page(s) 101 - 111
DOI 10.1051/ebr/2009006
Published online 10 July 2009

Environ. Biosafety Res. 8 (2009) 101-111
DOI: 10.1051/ebr/2009006

In-field frequencies and characteristics of oilseed rape with double herbicide resistance

Antje Dietz-Pfeilstetter and Peter Zwerger

Institute for Biosafety of Genetically Modified Plants and Institute for Plant Protection in Field Crops and Grassland, Julius Kühn-Institut, Federal Research Centre for Cultivated Plants, Messeweg 11-12, 38104 Braunschweig, Germany

Received November 6, 2008; Accepted May 13, 2009; published online 10 July 2009

Abstract - When growing different transgenic herbicide-resistant oilseed rape cultivars side by side, seeds with multiple herbicide resistance can arise, possibly causing problems for the management of volunteer plants. Large-scale field experiments were performed in the years 1999/2000 and 2000/2001 in order to investigate the frequencies and the consequences of the transfer of herbicide resistance genes from transgenic oilseed rape to cultivars grown on neighboring agricultural fields. Transgenic oilseed rape with resistance to glufosinate-ammonium (LibertyLink, LL) and with glyphosate resistance (RoundupReady, RR), respectively, was sown in adjacent 0.5 ha plots, surrounded by about 8 ha non-transgenic oilseed rape. The plots and the field were either in direct contact (0.5 m gap width) or they were separated by 10 m of fallow land. Seed samples taken during harvest in the transgenic plots at different distances were investigated for progeny with resistance to the respective other herbicide. It was found that outcrossing frequencies were reduced to different extents by a 10 m isolation distance. In addition to pollen-mediated transgene flow as a result of outcrossing, we found considerable seed-mediated gene flow by adventitious dispersal of transgenic seeds through the harvesting machine. Volunteer plants with double herbicide resistance emerging in the transgenic plots after harvest were selected by suitable applications of the complementary herbicides Basta $^{\circledR}$ and Roundup Ultra $^{\circledR}$. In both years, double-resistant volunteers were largely restricted to the inner edges of the plots. Expression analysis under controlled laboratory conditions of double-resistant plants generated by manual crosses revealed stability of transgene expression even at elevated temperatures. Greenhouse tests with double-resistant oilseed rape plants gave no indication that the sensitivity to a range of different herbicides is changed as compared to non-transgenic oilseed rape.


Key words: herbicide resistance / Brassica napus / gene flow / gene expression / gene stacking / volunteer control

Corresponding author: antje.dietz@jki.bund.de

© ISBR, EDP Sciences 2009


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