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Issue Environ. Biosafety Res.
Volume 5, Number 2, April-June 2006
Page(s) 111 - 117
DOI 10.1051/ebr:2007001
Published online 01 March 2007

Environ. Biosafety Res. 5 (2006) 111-117
DOI: 10.1051/ebr:2007001

Tracing field hybridization in Ryegrass species using microsatellite and morphological markers

Eimear Ryan1, Ewen Mullins2, James Burke2, Martin Downes1 and Conor Meade1

1  Molecular Ecology Laboratory, Institute of Bioengineering and Agroecology, National University of Ireland, Maynooth, Co. Kildare, Ireland
2  Teagasc Crop Research Centre, Oak Park, Carlow, Co. Carlow, Ireland

(Received June 29, 2006; accepted December 24, 2006; published online 1 March 2007)

Abstract - We have assessed the utility of morphological and microsatellite markers for tracing field hybridization between Lolium multiflorum and Lolium perenne in cereal-enclosed gene flow plots. The presence of awns on the inflorescence of F1 hybrids was found to be a reliable, but underscoring, indicator of L. multiflorum paternity in L. perenne derived seed as determined by inheritance of species-specific alleles at the microsatellite locus 'H01 H06' in these progeny. A positive correlation was evident in the experimental treatment between the number of pollen donor plants in a given plot and the frequency of hybrid F1 seed harvested from pollen receptor plants in that plot. These experiments have established the utility of naturally occurring heritable markers for the measurement of gene flow rates in field Ryegrass populations, with particular significance for risk assessment modeling of potential gene flow from transgenic grass cultivars.


Key words: Lolium perenne / Lolium multiflorum / pollen flow / gene flow / molecular markers / risk assessment

Corresponding author: Conor Meade conor.v.meade@nuim.ie

© ISBR, EDP Sciences 2007


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