Lack of stable inheritance of introgressed transgene from oilseed rape in wild radish

Free access article

Issue		Environ. Biosafety Res. Volume 3, Number 4, October-December 2004


Page(s)		209 - 214
DOI		10.1051/ebr:2005007

Environ. Biosafety Res. 3 (2004) 209-214
DOI: 10.1051/ebr:2005007

Lack of stable inheritance of introgressed transgene from oilseed rape in wild radish

Anoir Al Mouemar¹ and Henri Darmency²

¹ Department of Plant Protection, Faculty of Agronomy, Damas University, Syria
² Unité Mixte de Recherche sur la Biologie et la Gestion des Adventices, INRA, BP 86510, 21065 Dijon, France

(Received December 6, 2004; accepted March 17, 2005)

Abstract
Hybridization of Brassica napus L. (oilseed rape) and Raphanus raphanistrum L. (wild radish) has been demonstrated, and may be the first step towards introgression of transgenes in this wild relative. If wild radish were to display a new adaptive advantage by expressing the transgene, this could modify the ecological balance of species within the agro-ecosystem. To determine if transgenes remained stable in the hybrid, the frequency of herbicide resistance was studied over four advanced generations of hybrid progeny (G8 to G11) that were subjected to herbicide selection pressure. It is expected that hemizygous resistant plants containing an herbicide resistance transgene back-crossed to wild radish would have 50% resistant progeny. In each of the G8 to G11 generations, only 18% of the progeny from resistant plants were resistant. The chromosome complement of herbicide-susceptible progenies, analyzed at G9, was not different from that of wild populations of wild radish. Herbicide-resistant G9 progeny showed higher chromosome instability, and one third of the progeny contained a supernumerary chromosome. These results suggest that in the presence of herbicide selection pressure, the transgene for herbicide resistance would be maintained despite a lack of stabilized introgression. In the absence of selection, the frequency of resistance in the population is expected to decline.

Key words: transgene / herbicide resistance / inheritance / hybridization / introgression / chromosome / Brassica napus / Raphanus raphanistrum

Corresponding author: Henri Darmency Darmency@dijon.inra.fr

© ISBR, EDP Sciences 2004

What is OpenURL?

The OpenURL standard is a protocol for transmission of metadata describing the resource that you wish to access. An OpenURL link contains article metadata and directs it to the OpenURL server of your choice. The OpenURL server can provide access to the resource and also offer complementary services (specific search engine, export of references...). The OpenURL link can be generated by different means.

If your librarian has set up your subscription with an OpenURL resolver, OpenURL links appear automatically on the abstract pages.
You can define your own OpenURL resolver with your EDPS Account. In this case your choice will be given priority over that of your library.
You can use an add-on for your browser (Firefox or I.E.) to display OpenURL links on a page (see http://www.openly.com/openurlref/). You should disable this module if you wish to use the OpenURL server that you or your library have defined.